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一种快速通过对16S RNA基因限制性片段长度多态性进行分析的鉴定菌种的两步法已建立,并被用于鉴定属于弯曲菌、弓形菌和螺杆菌的分离菌株。有158份标本(26例对照标本和132例临床分离标本)已被成功鉴定。 PCR-RFLP使用的是纯化的DNA和原始的细菌细胞溶解产物。研究中的所有细菌均通过共26个一套引物和一个限制性核酸内切酶实现基因水平的鉴定。由于一些菌株
A two-step method for rapid identification of strains by analysis of 16S RNA gene restriction fragment length polymorphisms has been established and used to identify isolates belonging to Campylobacter, Toxoplasma and Helicobacter species. A total of 158 specimens (26 controls and 132 clinically separated specimens) have been successfully identified. PCR-RFLP uses purified DNA and the original bacterial lysate. All of the bacteria in the study identified the genomic level through a total of 26 sets of primers and a restriction endonuclease. Due to some strains