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目的:通过建立大鼠心肌梗死模型,以流式细胞和免疫组化等方法探讨硝酸异山梨酯通过20-HETE调控内皮祖细胞促进血管新生的能力。方法:通过结扎冠脉前降支构建大鼠心肌梗死模型并给予ISDN及20-HETE特异性抑制剂(HET0016)干预8周,观察梗死面积、梗死交界区微血管密度及循环血中EPCs的数量。结果:ISDN有助于缩小心梗面积,增加梗死交界区微血管密度及循环血中的EPCs数量,20-HETE特异性抑制剂可部分阻断ISDN的效应。结论:ISDN可通过20-HETE调控EPCs促进血管新生。
OBJECTIVE: To establish a myocardial infarction model in rats by flow cytometry and immunohistochemistry to investigate the ability of isosorbide dinitrate to regulate angiogenesis by regulating endothelial progenitor cells through 20-HETE. Methods: The model of myocardial infarction was established by ligating the anterior descending coronary artery and the intervention of ISDN and 20-HETE specific inhibitor (HET0016) for 8 weeks. The area of infarction, the density of microvessels at infarct junction area and the number of circulating EPCs were observed. RESULTS: ISDN helped to reduce infarct size, increase microvessel density in the infarct junction and the number of circulating EPCs. 20-HETE-specific inhibitors partially blocked the effect of ISDN. Conclusion: ISDN can promote angiogenesis through the regulation of EPCs by 20-HETE.