本文介绍了一种较新的分离蛋白质类的快速方法,即快速蛋白液相层析(FPLC)法。采用对蛋白质有高效能的亲水性聚醚所组成的凝胶,在中等压力下达到快速高效分离生物分子如蛋白质、酶、多肽及多核苷酸等。作者介绍了FPLC仪器及实验条件。用此法快速、简便、适于分离肾衰竭病人尿中血清蛋白,可应用于临床研究。 This article describes a relatively new rapid method for the isolation of proteins, the Rapid Protein Liquid Chromatography (FPLC) method. The gel is made of hydrophilic polyether with high performance for protein, and can quickly and efficiently separate biological molecules such as proteins, enzymes, peptides and polynucleotides under moderate pressure. The author introduced the FPLC instrument and experimental conditions. This method is fast, simple, suitable for the separation of urinary serum protein in patients with renal failure, can be used in clinical studies.