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为了探明水稻土落干过程对温室气体排放和反硝化微生物的影响,通过模拟水稻土淹水落干过程,系统监测了落干开始后24 h内N2O的释放和氧化还原电位(Eh)的变化,并利用实时PCR(qPCR)方法测定了反硝化功能基因narG和nosZ的丰度.结果表明:落干开始后4 h N2O释放量就明显增加,在24 h时N2O的释放量比淹水对照增加了5倍多;narG和nosZ基因丰度也随着落干过程的推移而快速增加;而且N2O排放通量与narG基因呈极显著相关(P<0.01).表明水稻土短期淹水落干过程中,含narG基因反硝化微生物是驱动N2O释放的主要功能微生物.
In order to investigate the effects of soil drying on greenhouse gas emissions and denitrifying microorganisms, N2O release and redox potential (Eh) were monitored systematically by simulating flooding and drying process of paddy soil. , And the abundance of denitrification gene narG and nosZ was measured by real-time PCR (qPCR) method.The results showed that the release of N2O increased significantly at 4 h after the beginning of the dry-out, and the release of N2O at 24 h was more than that of the flooded control And the abundance of narG and nosZ also increased rapidly with the desiccation process, and the N2O flux was significantly correlated with the narG gene (P <0.01), indicating that during the short-term flooding and drying of paddy soil , DenG containing narG gene is the main function of microorganisms that drive N2O release.