论文部分内容阅读
目的目前临床上检测肺炎支原体的微量颗粒凝集法,存在敏感性不高、受时间限制的缺点,本文采用nested PCR方法,扩增16S rRNA基因,试图建立一种敏感性高、特异性强的肺炎支原体检测方法。方法收集256例疑似肺炎支原体感染患儿的咽拭子,采用nested PCR法扩增16S rRNA基因,对比微量颗粒凝集法及传统PCR法检测结果,进行统计学分析。结果 nested PCR法与微量颗粒凝集法对比有早期诊断的特点,nested PCR法与传统PCR法对比,具有较高的准确度、敏感性和阴性预测值。结论 nested PCR法更适合临床应用。
Objective To detect Mycoplasma pneumoniae by microparticle agglutination, which is not sensitive and time-limited. In this paper, 16S rRNA gene was amplified by nested PCR in order to establish a highly sensitive and specific pneumonia Mycoplasma detection method. Methods Throat swabs were collected from 256 children suspected of Mycoplasma pneumoniae infection. The 16S rRNA gene was amplified by nested PCR, and compared with the results of microparticle agglutination and traditional PCR, the data were analyzed statistically. Results The nested PCR method had the characteristics of early diagnosis compared with the microparticle agglutination method. Compared with the traditional PCR method, the nested PCR method had higher accuracy, sensitivity and negative predictive value. Conclusion nested PCR is more suitable for clinical application.