本文以原代培养的人脐静脉内皮细胞(HUVEC)为材料,研究了肿瘤坏死因子(TNF)对HUVEC的损伤作用及循环内皮细胞(CEC)的形成机制。实验结果表明,TNF能造成HUVEC大片脱落,并出现了与CEC极为相似的形态。同时,TNF明显破坏了纤溶酶原激活剂抑制物活性。结果表明,TNF的直接细胞毒作用可致HUVEC转变成CEC的细胞形态,TNF浓度增加可能是病理情况下CEC增多的重要原因。 In this paper, the primary cultured human umbilical vein endothelial cells (HUVEC) were used to study the injury of tumor necrosis factor (TNF) to HUVEC and the formation mechanism of circulating endothelial cells (CEC). The experimental results show that TNF can cause massive HUVEC shedding, and appeared very similar to CEC morphology. At the same time, TNF significantly disrupts plasminogen activator inhibitor activity. The results show that the direct cytotoxicity of TNF can lead to HUVEC into CEC cell morphology, TNF concentration may be an important reason for the increase of CEC in pathological conditions.