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目的 研究新生儿缺氧缺血性脑病 (HIE)患者血清和脑脊液中神经元特异性烯醇化酶 (NSE)水平的变化及其临床意义。方法 对 40例HIE患儿和 10例同期无窒息、无神经系统疾病的足月新生儿出生后第 3天常规留取股静脉血和脑脊液各 1ml,迅速低温离心后取上清液用酶联免疫法测定NSE ,对 34例随访者采用盖氏法测定其发育商。结果 HIE患儿血清和脑脊液中NSE的浓度明显高于对照组 (P <0 .0 1) ,随着HIE临床分度的增加 ,NSE的含量显著升高 ,并且血清和脑脊液NSE的变化具有较好的相关性。结论 NSE可作为早期判断HIE脑损伤程度和预后的生化指标。在判断HIE脑损伤程度及预后时血清NSE水平与脑脊液NSE值有同等价值
Objective To study the changes and clinical significance of neuron specific enolase (NSE) in serum and cerebrospinal fluid (CSF) of neonates with hypoxic-ischemic encephalopathy (HIE). Methods 40 infants with HIE and 10 infants with full term infants without asphyxia and no neurological diseases were enrolled in this study. On day 3 postnatal, 1 ml each of femoral vein and cerebrospinal fluid was collected and centrifuged at low temperature. Immunoassay was used to measure NSE, 34 patients were followed up by Gai’s method. Results The serum levels of NSE in serum and cerebrospinal fluid of children with HIE were significantly higher than those in the control group (P <0.01). The levels of NSE in serum and cerebrospinal fluid were significantly increased with the increase of HIE clinical grade, and the changes of NSE in serum and cerebrospinal fluid Good relevance. Conclusion NSE can be used as an early biochemical marker to judge the degree and prognosis of HIE brain injury. In determining the degree of HIE brain injury and prognosis serum NSE levels and CSF NSE value has the same value