目的探讨子宫肌层组织内SULT2A1及SULT1E1的表达水平与子宫肌瘤发病的关系。方法选取2015年1月-2016年10月于沧州市沧县医院因患子宫肌瘤而行子宫全切除术或子宫次全切除术患者32例为子宫肌瘤组,并选取同年龄段的15例因子宫脱垂行子宫切除术患者作为对照组,采用化学发光分析法分别测定所有纳入患者血清及组织匀浆中雌二醇(E_2)、孕酮(P)含量,并以Western印迹方法检测SULT2A1蛋白的表达,最后采用Pearson相关分析分析组间子宫肌瘤发病与子宫肌层组织内SULT2A1及SULT1E1的相关性。结果 (1)肌瘤组织E_2、P含量显著高于肌瘤包膜外子宫肌层组及子宫脱垂组,肌瘤组血清E_2、P含量明显低于健康女性组(P<0.05);(2)SULT2A1、SULT1E1在肌瘤包膜外肌层组织、肌瘤组织、正常子宫肌层中表达存在显著差异(P<0.05),具体表现为膜外肌层组织<肌瘤组织<正常子宫肌层;(3)皮尔逊相关性分析显示,肌瘤组织SULT2A1、SULT1E1与相应组织组织匀浆中E_2、P水平呈显著负相关(P<0.05),而与血清中E_2、P水平无明显相关性。结论子宫肌瘤的发生、发展与子宫肌瘤组织局部SULT2A1、SULT1E1蛋白活性降低有关。 Objective To investigate the relationship between the expression of SULT2A1 and SULT1E1 in myometrium and the incidence of uterine myoma. Methods From January 2015 to October 2016 in Cangzhou City, Cangxian Hospital due to uterine fibroids hysterectomy or subtotal hysterectomy patients 32 cases of uterine fibroids, and select the same age group of 15 Cases of uterine prolapse hysterectomy patients as a control group, the chemiluminescence assay was used to measure the inclusion of serum and tissue homogenate in patients with estradiol (E2), progesterone (P) content, and detected by Western blot SULT2A1 protein expression, and finally using Pearson correlation analysis between the incidence of uterine fibroids and myometrial tissue SULT2A1 and SULT1E1 correlation. Results (1) The contents of E_2 and P in myoma tissue were significantly higher than those in uterine myometrium and uterine prolapse group. The levels of E_2 and P in myoma group were significantly lower than those in healthy women (P <0.05). ( 2) The expression of SULT2A1 and SULT1E1 in myometrial extracapsular myometrium, myoma and normal myometrium were significantly different (P <0.05), especially in the myometrium