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为探讨丙型肝炎病毒（ＨＣＶ）结构基因在ＨＣＶ感染中的致病性，构建了中国丙型肝炎病毒５ＵＴＲ区与结构基因区（Ｃ＋Ｅ１＋Ｅ２）的表达质粒，并通过显微注射法将其接种入小鼠受精卵内制备转基因小鼠。共注射受精卵４１０枚，存活３１２枚，植入后产仔６０只；转基因鼠尾部组织ＰＣＲ法ＤＮＡ检测证明有靶基因的整合；转基因小鼠的肝、肾、脾、心、肺、小肠、血中均有靶基因的转录，而在脑组织中无转录。３只Ｇｏ代整合小鼠经与正常ＩＣＲ小鼠配种，获Ｇ１代小鼠３２只，其中１３只有ＨＣＶ结构基因的整合。这一结果表明，ＨＣＶ结构基因转基因小鼠动物模型已建成。
In order to investigate the pathogenicity of hepatitis C virus (HCV) structural gene in HCV infection, we constructed expression plasmids of 5UTR region and structural gene region (C + E1 + E2) of hepatitis C virus in China and inoculated them by microinjection Transgenic mice were prepared in mouse fertilized eggs. Co-injection of fertilized eggs 410, survived 312, after implantation, 60 litters; transgenic tail tissue PCR DNA test showed that target gene integration; transgenic mice liver, kidney, spleen, heart, lung, small intestine, The target gene transcription in the blood, but not in the brain tissue transcription. Three Go-generation mice were crossed with normal ICR mice to obtain 32 G1 mice, of which 13 had HCV structural gene integration. This result indicates that the animal model of HCV structural gene transgenic mice has been established.