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目的 探讨我国睑结膜部炎症者病灶部是否存在肺炎衣原体 (Cpn)。 方法 收集 39例慢性结膜炎、沙眼患者的睑结膜病灶部刮取物 ,应用nestedPCR(nPCR)技术检测肺炎衣原体 (Cpn)和沙眼衣原体 (Ct)核酸。并随机抽取 2例CpnnPCR阳性产物进行全自动DNA测序 ,加以佐证。 结果 39例患者中Cpn阳性 13例 ( 33 3% ) ,Ct阳性 5例( 12 8% )。慢性结膜炎和沙眼病例均有Cpn和Ct阳性者。 2例CpnnPCR阳性产物经全自动DNA测序与Cpn(CWL—2 9株 )高度同源。结论 我国睑结膜部炎症者病灶部存在Cpn。
Objective To investigate the presence or absence of Chlamydia pneumoniae (Cpn) in the lesions of the palpebral conjunctival inflammation in China. Methods 39 patients with chronic conjunctivitis and trachoma were collected with palpebral conjunctiva lesions. Nucleic acid of Cpn and Ct were detected by nested PCR (nPCR). And randomly selected two cases of CpnnPCR positive products for automatic DNA sequencing to be confirmed. Results Of 39 patients, Cpn was positive in 13 cases (33.3%) and Ct was positive in 5 cases (12.8%). Chronic conjunctivitis and trachoma cases have Cpn and Ct positive. Two CpnnPCR positive products were highly homologous to Cpn (CWL-2 9) by automated DNA sequencing. Conclusion There are Cpn in the lesions of the palpebral conjunctival inflammation in China.