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目的:考察龙须藤的最佳乙醇提取浓度,并以硅胶柱色谱法分离得到龙须藤总黄酮,对分离得到的龙须藤总黄酮进行含量测定。方法:采用药效学跟踪试验,参考龙须藤不同浓度乙醇提取物对膝关节炎症模型大鼠的抗炎作用,选取龙须藤的最佳乙醇提取浓度,采用硅胶柱色谱方法从龙须藤乙醇提取物的乙酸乙酯萃取部位分离得到龙须藤总黄酮,HPLC对分离得到的龙须藤总黄酮进行含量测定。结果:龙须藤的70%乙醇提取物抗炎作用最好,采用溶剂系统为二氯甲烷-乙酸乙酯(8∶1~4∶1)梯度洗脱分离得到的龙须藤总黄酮中3’,4’-亚甲二氧基-5’,5,6,7-四甲氧基黄酮、3’,4’,5’,5,7-五甲氧基黄酮含量分别为21.3%,46.7%。结论:龙须藤的最佳乙醇提取浓度为70%,溶剂系统为二氯甲烷/乙酸乙酯(8/1~4/1)梯度洗脱分离龙须藤总黄酮的方法可行。
OBJECTIVE: To investigate the optimum concentration of ethanol extracted from Gracilaria tenuistipitata and to extract the total flavonoids of Gracilaria tenuistifolia by silica gel column chromatography. The content of total flavonoids in Gracilaria tenuistipitata was determined. Methods: The pharmacodynamic tracing test was used to study the anti-inflammatory effect of ethanol extract of Radix et Rhizoma with different concentration on the inflammation model of knee joint. The optimal concentration of ethanol extract was determined by silica gel column chromatography. Ethanol extract of the ethyl acetate extract parts were isolated from the total flavonoids longan, HPLC isolated from the content of the total flavonoids were determined. Results: The anti-inflammatory effect of 70% ethanol extract of Gracilaria tenuistifolia was the best. The solvent was extracted by gradient elution from dichloromethane to ethyl acetate (8: 1 ~ 4: 1) ’, 4’-methylenedioxy-5’, 5,6,7-tetramethoxyflavone, 3 ’, 4’, 5 ’, 5,7-pentamethoxyflavone were 21.3% 46.7%. CONCLUSION: The optimal ethanol extraction concentration is 70%. The solvent system is suitable for the separation of the total flavonoids from Glyptosternophilus tenuis with dichloromethane / ethyl acetate (8/1 ~ 4/1) gradient.