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目的建立测定大鼠血浆中丁香酚的HPLC法。方法流动相为甲醇-0.5%冰醋酸(63∶37),采用Allsphere ODS-2RP-18色谱柱(250 mm×4.6 mm,5μm),流速0.8 mL.min-1,检测波长280 nm,柱温30℃。结果丁香酚0.0895～11.445μg.mL-1与峰面积比的线性关系良好,最低定量浓度为0.0895μg.ml-1,3种浓度的平均回收率为98.4%～106.5%,日内RSD≤6.65%,日间RSD≤6.78%,3种浓度的萃取回收率为93.76±3.6%、101.37±1.4%、98.83±0.6%(n=3)。结论所建方法简单、灵敏、准确可靠,可用于丁香酚的药动学研究。
Objective To establish a HPLC method for the determination of eugenol in rat plasma. The mobile phase consisted of methanol-0.5% glacial acetic acid (63:37). The mobile phase consisted of an ODS-2 RP-18 column (250 mm × 4.6 mm, 5 μm) with a flow rate of 0.8 mL · min- 30 ° C. Results The calibration curves of eugenol 0.0895 ~ 11.445μg.mL-1 showed a good linear relationship with the peak area ratio, with the lowest concentration of 0.0895μg.ml-1, the average recovery of 1,3-glucan was 98.4% -106.5%, RSD≤6.65% , Daytime RSD≤6.78%. The extraction recoveries of the three concentrations were 93.76 ± 3.6%, 101.37 ± 1.4% and 98.83 ± 0.6% (n = 3), respectively. Conclusion The method is simple, sensitive, accurate and reliable and can be applied to the pharmacokinetics of eugenol.