论文部分内容阅读
目的探讨静脉输注的真皮多能干细胞(dermal multipotent stemcells,dMSCs)向放创复合伤大鼠创面优势分布的机制。方法RT-PCR和免疫组织化学检测创面中基质细胞源性细胞因子(stromal-dereivedfactor-1,SDF-1)的表达水平,以及dMSCs中SDF-1受体CXCR4的表达水平。Transwell培养体系中,观察SDF-1对dMSCs的趋化作用。结果放创复合伤大鼠创面SDF-1的表达水平明显高于正常大鼠,同时dMSCs表达CXCR4。体外趋化实验发现,SDF-1对dMSCs有很强的趋化作用,而使用SDF-1的中和性抗体后,放创复合伤大鼠伤口液对dMSCs的趋化作用明显下降(P<0·05)。结论放创复合伤大鼠创面上调表达的SDF-1在移植dMSCs向创面的优势分布中有重要作用。
Objective To investigate the mechanism of the superiority distribution of intravenous infusion of dermal multipotent stem cells (dMSCs) to the wounds of rats with combined wounds. Methods The expression of stromal-dereived factor-1 (SDF-1) and the expression of CXCR4 on SDF-1 in dMSCs were detected by RT-PCR and immunohistochemistry. Transwell culture system, SDF-1 on the dMSCs chemotaxis. Results The expression of SDF-1 in wounds of rats with combined injury was significantly higher than that in normal rats, meanwhile, the expression of CXCR4 in dMSCs was higher than that in normal rats. In vitro chemotaxis assay showed that SDF-1 had a strong chemotactic effect on dMSCs. However, the chemotactic effect of SDF-1-neutralizing antibody on wound healing of dMSCs was significantly decreased (P < 0 · 05). Conclusion Up-regulated expression of SDF-1 in wounds of wounds in combination may play an important role in the distribution of dMSCs to the predominant wounds.