论文部分内容阅读
应用共聚焦显微镜和流式细胞仪双荧光标记法 ,体外研究不同转移性肺癌细胞与血管内皮细胞的粘附性 ,并检测药物对上述过程的影响。结果显示 ,高转移性肺癌细胞 (PG)与血管内皮细胞的粘附能力、CD4 4和CD5 4表达水平明显高于低转移性的肺癌细胞(D9) ;当血管内皮细胞被脂多糖激活后 ,PG细胞与其的粘附性较激活前明显增加 ;茶多酚对PG细胞与血管内皮细胞的粘附性具有明显的抑制作用。提示粘附分子在介导肺癌细胞与内皮细胞粘附及血行转移中起着十分重要的作用 ,茶多酚具有抗肿瘤转移作用
The confocal microscopy and flow cytometry double fluorescent labeling method were used to study the adhesion of different metastatic lung cancer cells and vascular endothelial cells in vitro and the influence of drugs on the above process was tested. The results showed that the adhesion ability of high metastatic lung cancer cells (PGs) to vascular endothelial cells, and the expression of CD4 4 and CD54 were significantly higher than those of low metastatic lung cancer cells (D9). When the vascular endothelial cells were activated by lipopolysaccharide, PG cells and their adhesion was significantly increased before activation; tea polyphenols on PG cells and vascular endothelial cell adhesion has obvious inhibitory effect. Suggesting that adhesion molecules play a very important role in mediating adhesion of lung cancer cells and endothelial cells and hematogenous metastasis. Tea polyphenols have anti-tumor metastasis effects