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目的:探讨抑癌基因蛋白PTEN和凋亡抑制基因蛋白Survivin在宫颈癌变过程中的联合表达。结合宫颈病变的临床病理学特征,对两者基因蛋白阳性表达的相关性进行临床研究。方法:选取20例宫颈糜烂伴鳞状上皮化生、30例宫颈上皮内瘤变以及49例宫颈鳞状细胞癌患者,应用免疫组化SP法检测各组宫颈组织中抑癌基因蛋白PTEN和凋亡抑制基因蛋白Survivin的表达。选取10例正常宫颈组织作为对照组。结果:①在宫颈癌变动态过程中,从宫颈糜烂伴鳞状上皮化生、宫颈上皮内瘤变到宫颈鳞状细胞癌,抑癌基因PTEN的表达逐渐减弱(P<0.05)。而凋亡抑制基因蛋白Survivin表达则逐渐增强(P<0.05)。②PTEN在宫颈癌中的阳性表达与临床分期、病灶大小有关(P<0.05),淋巴结转移组中其阳性表达率明显低于无淋巴结转移组(P<0.01),与肿瘤分化程度、年龄无关(P>0.05)。③Survivin在宫颈癌中的阳性表达与临床分期、病灶大小和肿瘤分化程度有关(P<0.05),淋巴结转移组中其阳性表达率明显高于无淋巴结转移组(P<0.01),而与年龄大小无关(P>0.05)。④PTEN和Survivin在宫颈上皮内瘤变和宫颈癌中的阳性表达呈负相关。结论:抑癌基因PTEN、凋亡抑制基因Survivin在宫颈癌变过程中协同参与了宫颈癌的发生和进展。PTEN和Survivin在宫颈癌组织中阳性表达为其早期诊断和预后判断提供了新的评价指标,也为宫颈癌的基因治疗提供了新的生物学靶点。
Objective: To investigate the co-expression of tumor suppressor gene PTEN and apoptosis inhibitor protein Survivin in cervical carcinogenesis. Combined with the clinicopathological features of cervical lesions, the clinical research of the correlation between the two gene protein expression was carried out. Methods: Twenty cases of cervical erosion with squamous metaplasia, 30 cases of cervical intraepithelial neoplasia and 49 cases of cervical squamous cell carcinoma were selected. Immunohistochemical SP method was used to detect the expression of tumor suppressor gene PTEN and Expression of Survivin, a Subunit Inhibitor Protein. Ten cases of normal cervical tissue were selected as the control group. Results: (1) In the process of cervical carcinogenesis, the expression of tumor suppressor gene PTEN decreased gradually from cervical erosion with squamous metaplasia and cervical intraepithelial neoplasia to cervical squamous cell carcinoma (P <0.05). Survivin expression increased gradually (P <0.05). (2) The positive expression of PTEN in cervical cancer was related to the clinical stage and the size of the lesion (P <0.05). The positive expression rate of PTEN in cervical lymph node metastasis was significantly lower than that in non-lymph node metastasis (P <0.01) P> 0.05). (3) The positive expression of Survivin in cervical cancer was related to the clinical stage, the size of lesion and the degree of tumor differentiation (P <0.05). The positive expression rate of Survivin in lymph node metastasis group was significantly higher than that in no lymph node metastasis group (P <0.01) Not related (P> 0.05). ④ PTEN and Survivin in cervical intraepithelial neoplasia and cervical cancer in the positive expression was negatively correlated. CONCLUSION: The tumor suppressor gene PTEN and the apoptosis-inhibiting gene Survivin are involved in the carcinogenesis and progression of cervical cancer during cervical carcinogenesis. The positive expression of PTEN and Survivin in cervical cancer tissue provides a new evaluation index for their early diagnosis and prognosis, and also provides a new biological target for gene therapy of cervical cancer.