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用免疫组织化学技术使同一组织切片同时显示二种抗原,这将有助于了解不同抗原的定位及相互间关系。本文对辣根过氧化物酶法的不同显色方法及配伍作了摸索。 材料、方法和结果 一、组织:淋巴结活检标本,低温石蜡包埋,切片厚5μm。 二、抗血清:羊抗人k,羊抗人λ,酶联葡萄球菌A蛋白(SPA-P)(卫生部上海生物制品研究所产品)。 兔抗人k,兔抗溶菌酶(美国Dako Cor-
Using immunohistochemistry to slice the same tissue simultaneously shows both antigens, which will help to understand the positioning of different antigens and their relationships. In this paper, horseradish peroxidase method of different color and compatibility were explored. Materials, methods and results First, the organization: Lymph node biopsy specimens, embedded in low temperature paraffin, slice thickness 5μm. Second, the antiserum: sheep anti-human k, goat anti-human λ, Staphylococcus aureus enzyme protein (SPA-P) (Ministry of Health Shanghai Institute of Biological Products). Rabbit anti-human k, rabbit anti-lysozyme (the United States Dako Cor-