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目的 观察静脉注射用免疫球蛋白与免疫抑制剂偶联物对单核—巨噬细胞的杀伤作用 ,为其临床治疗ITP奠定实验基础。方法 分别用间接和直接交联法将IVIG与MTX制备成偶联物 ,用间接免疫荧光法检测偶联物Fc段结合活性 ,以Fc受体阳性的小鼠巨噬细胞和人单核细胞白血病细胞株U937为靶细胞 ,用MTT法测定偶联物的杀伤活性。结果 偶联物对巨噬细胞的杀伤作用明显大于游离MTX ;偶联物对Fc受体阳性细胞的杀伤作用明显大于Fc受体阴性细胞。间接偶联物IVIG HSA MTX杀伤作用明显高于直接偶联物IVIG MTX。结论 MTX抗体偶联物在体外对单核—巨噬细胞显示了相对特异的杀伤活性
Objective To observe the killing effect of conjugate of intravenous immunoglobulin and immunosuppressant on monocyte-macrophage, and lay an experimental foundation for its clinical treatment of ITP. Methods Indirect and direct cross-linking methods were used to prepare IVIG and MTX conjugates. Fc fragment-binding activity of the conjugates was detected by indirect immunofluorescence. Fc receptor-positive mouse macrophages and human monocytic leukemia The cell line U937 is a target cell, and the killing activity of the conjugate is measured by the MTT method. Results The killing effect of conjugate on macrophages was significantly greater than that of free MTX. The killing effect of the conjugates on Fc receptor positive cells was significantly greater than that of Fc receptor negative cells. The indirect conjugate IVIG HSA MTX killing effect was significantly higher than the direct conjugate IVIG MTX. Conclusion MTX antibody conjugates showed relatively specific killing activity on monocytes-macrophages in vitro