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目的对三种乳腺癌骨转移模型复制及其检测方法进行比较研究.方法选择BALB/cA-nu/nu雌性6-8周龄裸鼠24只,分为原位注射组、尾静脉注射组和左心室注射组3组,每组8只,分别注射0.2 mL人乳腺癌MDA-MB-435细胞悬液(2×107/mL);40 d后做X-ray、PET-CT检查;45 d后进行动物解剖,取影像学阳性部位送病理检查.结果PET-CT观察到骨侵蚀影像;肉眼见明显的肋骨连续性中断;病理检查结果显示胸廓肋骨部位有肿瘤细胞浸润;X-ray检查未发现明显影像学改变.左心室注射法骨转移率为75%,尾静脉注射法骨转移率为12.5%,原位注射法未见骨转移.结论左心室注射法复制乳腺癌骨转移模型,与另外两种方法相比,可以获得较高骨转移率,是复制乳腺癌骨转移模型较为理想的一种方法.
OBJECTIVE: To compare the replication and detection of three bone metastasis models of breast cancer.Methods Twenty-four female BALB / cA-nu / nu female 6-8 weeks old mice were divided into three groups: in situ injection group, tail vein injection group and The rats in the left ventricular injection group were injected with 0.2 mL of MDA-MB-435 cell suspension (2 × 107 / mL). After 40 days, they were examined by X-ray and PET-CT. The animals were dissected and the positive parts of the imaging were taken for pathological examination.Results PET-CT images of the bone erosion were observed, and the obvious continuity of the ribs was discernable in the naked eye. The pathological examination showed that the thoracic ribs had tumor cell infiltration. X-ray examination did not Found that significant imaging changes.Liver injection of bone turnover rate of 75%, tail vein injection of bone metastasis rate was 12.5%, in situ injection of bone metastasis.Conclusion Left ventricular injection of breast cancer bone metastasis model, and Compared with the other two methods, higher bone metastasis rate can be obtained, which is a more ideal method to replicate the bone metastasis model of breast cancer.