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[目的]探讨桦褐孔菌水提取物对STZ诱导的糖尿病大鼠糖异生和胆固醇合成关键酶表达水平的影响.[方法]制备STZ诱导的糖尿病大鼠模型,随机分为糖尿病模型组(阴性对照组)、阳性对照组、桦褐孔菌水提取物大、中、小剂量组.阳性对照组给予200mg/kg盐酸二甲双胍,桦褐孔菌水提取物给药组分别灌胃给予桦褐孔菌水提取物900,600,300mg/kg.每日1次,连续给药8周.实验前后测定大鼠空腹血糖、糖化血红蛋白、糖耐量及TG,TC,LDL-C,HDL-C等指标,检测肝组织PEPCK,G-6-PASE,HMG CoA还原酶蛋白表达水平.[结果]与阴性对照组比较,桦褐孔菌水提取物中、大剂量组大鼠空腹血糖、糖化血红蛋白,TC,TG及LDL-C水平显著降低(P<0.05,P<0.01),HDL-C水平增高(P<0.05,P<0.01),PEPCK,G-6-PASE及HMG CoA还原酶表达水平显著降低,且呈剂量依赖性.[结论]桦褐孔菌水提取物对STZ诱导的糖尿病大鼠具有较好的降低血糖和血脂水平的作用,其机制可能与降低PEPCK,G-6-PASE,HMG CoA还原酶的表达及减少糖异生和胆固醇合成过程有关.
[Objective] To investigate the effects of water extracts from Inonotus obliquus on the expression of key enzymes of gluconeogenesis and cholesterol synthesis in STZ-induced diabetic rats. [Methods] The diabetic rats induced by STZ were randomly divided into diabetic model group Negative control group), positive control group, water extract of Inonotus obliquus large, medium and small dose group.The positive control group was given 200mg / kg metformin hydrochloride, water extract of Inonotus obtia Fungus water extract 900,600,300 mg / kg once daily for 8 weeks.Fasting fasting blood glucose, glycosylated hemoglobin, glucose tolerance and TG, TC, LDL-C, HDL-C and other indicators were measured in rats before and after the experiment The expression of PEPCK, G-6-PASE and HMG CoA reductase in liver tissue were detected by ELISA. [Results] Compared with the negative control group, the fasting blood glucose, glycosylated hemoglobin, TC, TG (P <0.05, P <0.01), HDL-C level increased (P <0.05, P <0.01), PEPCK, G-6-PASE and HMG CoA reductase levels decreased significantly In a dose-dependent manner. [Conclusion] Water extract from Inonotus obliquus had better blood glucose and lipid lowering effect on STZ-induced diabetic rats Action, which may be related decrease PEPCK, G-6-PASE, HMG CoA reductase enzyme and reducing gluconeogenesis and cholesterol synthesis processes.