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为筛选新型高效油菜复配化学杂交剂,研究其应用技术及杀雄机理,用其拟制细胞质不育系低温微粉和选育化杀两系杂交种.将2种高活性杂交剂复配,多年多点大田试验研究其应用技术,生理生化、大田调查和细胞学观察研究其杀雄机理和杀雄效果,醋酸洋红染色测定花粉活力.油菜单核期在茎叶上喷雾0.5~0.8mg/L复配化学杂交剂,杀雄效果好,花药皱缩干瘪呈针状、花丝明显缩短、花药无花粉或为空秕、畸形的败育花粉,绒毡层提前解体或液泡化,全不育株率95%以上,不育株率100%,不育度95%以上,杂交种纯度提高8%以上.复配化学杂交剂是一种活性高、用量少、成本低、较为理想的新型油菜化学杂交剂.
In order to screen a new type of high efficient rapeseed compound chemical hybridization agent, study its application technology and killing mechanism, use it to prepare low temperature micropowder of cytoplasmic male sterile line and select and kill two-line hybrid seed, For many years, field experiments were carried out to study the mechanism of its killing and the effect of killing male mice by physiological, biochemical, field investigation and cytological observation, and the pollen viability was determined by the magenta staining of acetic acid.The spray of 0.5-0.8 mg / L composite chemical hybridization agent, killing effect is good, the anthers wrinkled dry needle-like, filament significantly shortened, anther pollen or empty 秕, abnormal abortion pollen, tapetum early disintegration or vacuolization, all infertility Strain rate of more than 95%, sterile plant rate of 100%, sterility more than 95%, hybrid purity increased more than 8% compound chemical hybridization agent is a high activity, less dosage, low cost, the ideal new type Rape chemical hybridization agent.