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胃癌是我国最常见的恶性肿瘤。其发生是以多个原癌基因的激活和肿瘤抑制基因的失活为基础的。P_(53)基因是一种人体内重要的细胞生长调节基因。定位在染色体17P13.1。它的突变在许多常见恶性肿瘤发展中起着重要作用,并且突变后的P_(53),基因起癌基因的作用,P_(53)基因突变的热点多集中在5、6、7、8外显子。因此,我们采用多聚酶反应单链构象多态(PCR-SSCP)分析对20例原发性胃腺癌胃镜活检组织7、8外显子突变进行了研究。以探讨P_(53)基因突变和人类胃癌发生的关系。
Gastric cancer is the most common malignancy in China. Its occurrence is based on the activation of multiple oncogenes and the inactivation of tumor suppressor genes. The P_53 gene is an important cell growth regulator gene in the human body. Located on chromosome 17P13.1. Its mutations play an important role in the development of many common malignancies, and mutations P_ (53), gene oncogenes, P_ (53) mutations are mostly focused on 5, 6, 7 and 8 Exon. Therefore, we used polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis to study the mutation of exon 7 and exon 8 of gastroscope biopsies in 20 patients with primary gastric adenocarcinoma. To investigate the relationship between P_53 gene mutation and the occurrence of human gastric cancer.