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正常人背上腺组织块经O.25%胰酶消化后置于25ml培养瓶中,加入DMEM(含IO%胎牛血清)后放在37C、C()2培养箱中培养,经过9d培养后进行药物实验。将培养的肾上腺组织随机分为3组:对照组(6瓶)每瓶加入15oplo.9%NaCI溶液;实验组分为氯化钙组(8瓶,每瓶浓度为SXIO-’m
Normal back to the gland tissue block by O. 25% Trypsin digestion placed in 25ml flasks, DMEM (containing 10% fetal bovine serum) and then placed in 37C, C () 2 incubator cultured after 9d after the drug test. The cultured adrenal tissue was randomly divided into 3 groups: the control group (6 bottles) added 15oplo per bottle. 9% NaCI solution; the experimental component is calcium chloride group (8 bottles, the concentration of each bottle is SXIO-mm