The molecular cloning of the G-1 genome segment of the Z-{10} strain of Hantaan virus and analysis of the first gene data on the currently widely used Hantavirus vaccine strain in China are presented. The G-1 genome segment of Z-{10} virus was amplified by the method of RT PCR, and the products were cloned into the pGEM|T vector after identification and purification. The clone was sequenced by Sangers dideoxy chain termination method. The 1449 bases of the Z-{10} G-1 genome segment ,and the coding for 483 amino acids were determined. The base compositions for the G-1 segment RNA determined from cDNA sequence information , were 20.6% A, 21.6% G, 18.8% C and 30.0% U. These values are similar to those of the 76/118, Lee and SR virus. As compared to the Z-{10} G-1 segment, the sequence homology at the nucleotide level is 87%(76/118, type Ⅰ), 86% (Lee, type Ⅰ),86% (Hojo, type Ⅰ), 67%(R22,type Ⅱ), and 59%(K22, type Ⅲ).The Z-{10} virus G-1 protein amino acid sequence identity with other Hantaan viruses (94-95% homology) was higher than that with Seoul type viruses (77-80%). More amino acid sequence heterogeneity between the Z-{10} and 76/118 was observed in the N terminal, especially the diverging cluster of ten amino acids at position 84 to 93 of the Z-{10} virus G-1 protein. Conclusion:(1) The Z-{10} strain is one of the Hantaan viruses. (2) The important region of the Z-{10} G-1 segment was conservative. (3) Although substantial divergence of nucleotide sequences of the G-1 genome segment was found between the Z-{10} strain and other type I Hantaviruses, relatively high amino acid sequence homology was shown among them. Thus, good immune protection could be obtained with the inactivated Meriones unguiculatus kidney cell vaccine against other strains of Hantaviruses. The molecular cloning of the G - 1 genome segment of the Z - {10 } strain of Hantaan virus and analysis of the first gene data on the currently most widely used Hantavirus vaccine strain in China are. The G - 1 genome segment of Z - {10 } virus was amplified by the method of RT PCR, and the products were cloned into the pGEM | T vector after identification and purification. The clone was sequenced by Sanger’s dideoxy chain termination method. The 1449 bases of the Z - {10 G - 1 genome segment, and the coding for 483 amino acids were determined. The base compositions for the G - 1 segment RNA determined from cDNA sequence information, were 20.6% A, 21.6% G, 18.8% C and 30.0% U. These values ​​are similar to those of the 76/118, Lee and SR virus. As compared to the The sequence homology at the nucleotide level is 87% (76/118, type I), 86% (Lee, type I), 86% (Hojo , type I), 67% (R22, type II), and 59% (K22, type III) .The Z - {10 } virus G - 1 protein amino acid sequence identity with other Hantaan viruses (94-95% homology) was higher than that with Seoul type viruses (77-80%). More amino acid sequence heterogeneity between the Z - {10 } and 76/118 was observed in the N terminal, especially the diverging cluster of ten amino acids at position 84 to 93 of the Z - {10 } virus G - 1 protein. Conclusion: (1) The Z - {10 } strain is one of the Hantaan viruses. (2) The important region of the Z - {10 } G - 1 segment was conservative (3) Although substantial divergence of nucleotide sequences of the G - 1 genome segment found between the Z - {10 } strain and other type I Hantaviruses, relatively high amino acid sequence homology was among Thus, good immune protection could be obtained with the inactivated Meriones unguiculatus kidney cell vaccine against other strains of Hantaviruses.