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实验使用组织病理学、活体压片和电镜技术,运用H.E常规染色法和美兰染色法对患有疑似类结节病的高体魳进行病理组织学诊断和观察。结果显示,患病高体魳肾脏和脾脏有大量白色小结节,直径大约1 mm;活体压片,观察到脾脏和肾脏中有散在短杆状细菌和大量呈团块状聚集的细菌结节;光学显微镜下,肾脏和脾脏有大量细菌聚集形成的嗜碱性结节,肾间质淤积的血液中也有大量细菌;透射电镜观察到肾脏和脾脏中有些巨噬细胞在吞噬细菌,有些菌体聚结成团形成结节,未见病毒颗粒。从病变组织中分离到两端极染的革兰氏阴性短杆菌,无鞭毛,长0.8～2.6μm,宽约0.6～1.2μm。患病高体魳器官主要组织病理学变化有肾小管上皮细胞变性坏死,肾间质淋巴细胞增生;脾淤血和淋巴细胞增生;肝细胞肿胀,脂肪变性。依组织病变、病原形态和特性,患病高体为类结节病。
Histopathology, live compression and electron microscopy were used in the experiment. Histopathology of the human body with suspected sarcoidosis was diagnosed and observed by H.E staining and methylene blue staining. The results showed that there were a large number of white nodules and a diameter of about 1 mm in the kidney and spleen of the diseased high body and the spleen. In vivo, there were scattered short rod-shaped bacteria in the spleen and kidney and a large number of bacterial nodules Under the light microscope, there were a large number of basophilic nodules formed in the kidney and spleen, and a large number of bacteria were also found in the blood stained by the renal interstitium. Some macrophages in the kidney and spleen were phagocytosed by transmission electron microscopy, Coalesced into a group formed nodules, no virus particles. Isolated from the diseased tissue of both ends of the extremely stained Gram-negative Brevibacterium, flagella, 0.8 ~ 2.6μm long and 0.6 ~ 1.2μm wide. Histopathological changes in the body of the main pathological changes of renal tubular epithelial cell degeneration and necrosis, interstitial lymphocyte proliferation; spleen congestion and lymphocyte proliferation; hepatocyte swelling, steatosis. According to tissue lesions, pathogen morphology and characteristics, the prevalence of elevated body-like sarcoidosis.