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【目的】了解内蒙古阿拉善和宁夏天然草原的小花棘豆和变异黄芪不同组织中内生真菌Undifilum oxytropis的显微分布特点和含量分布规律。【方法】通过石蜡切片结合乳酸酚棉蓝染色法观察,采用实时荧光定量PCR(RT-qPCR)进行定量研究,获得各组织(茎、叶、种子和根)中内生真菌分布和含量。【结果】种子中内生真菌主要定殖于种皮栅栏组织与薄壁组织两层的细胞间隙;叶片组织主要定殖于靠近气孔的表皮细胞层,茎髓中内生真菌围绕于茎髓质维管束纵轴边缘的薄壁细胞层中;RT-qPCR的检测限为0.029 pg/ng总DNA,各采样点相应组织内生真菌含量不同,两采样点小花棘豆种子中U.oxytropis含量均为最高,叶和茎相反,两地变异黄芪为种子中最高,根最低,叶和茎相反。【结论】内生真菌寄生在植物组织时对宿主组织和细胞类型均有选择性,生境对疯草中内生真菌的定殖和分布也有影响。
【Objective】 The aim of this study was to investigate the microscopic distribution and distribution of endophytic fungi Undifilum oxytropis in different tissues of Alnus cremastogyne and Astragalus mongholicus in Inner Mongolia. 【Method】 By means of paraffin section and lactate phenol cotton blue staining, quantitative analysis was carried out by real-time fluorescence quantitative PCR (RT-qPCR) to get the distribution and content of endophytic fungi in each tissue (stem, leaf, seed and root). 【Result】 The results showed that the endophytic fungi in seeds mainly colonized the intercellular space between the two layers of seed coat and palisade tissue. The leaf tissue mainly colonized the stratum astigmatism near stomata. The detection limit of RT-qPCR was 0.029 pg / ng total DNA, and the content of endophytic fungi in the corresponding tissues of each sampling point was different. The contents of U. oxytropis in both sampling points For the highest, leaves and stems on the contrary, the two mutant Astragalus is the highest seed, the lowest root, leaf and stem opposite. 【Conclusion】 Endophytic fungi are selective for both host tissues and cell types in plant tissues. Habitats also affect the colonization and distribution of endophytic fungi in wild plants.