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铁十字秋海棠 (Begoniamasoniana)和蟆叶秋海棠 (Begoniarex)幼叶外植体在MS +BA 2mg·L- 1+NAA 0 .2mg·L- 1培养基中 ,可以诱导出大量不定芽 ,将不定芽转移到1/ 2MS +IAA 0 .2~ 0 .3mg·L- 1或 1/ 2MS +NAA 0 .2mg·L- 1培养基中 ,芽体快速生长 ,叶片壮大 ,同时可以看到不定根的分化 ,如此得到生长健壮的试管苗 .将根芽健壮的试管苗出瓶移植 ,小苗正常发育 .多次移栽表明 ,保持小苗周围空气湿润 ,栽培基质通气良好是移栽成功的关键 .
In the medium of MS + BA 2 mg · L -1 + NAA 0.2 mg · L -1, young shoot explants of Begonia masoniana and Begonia rex could induce a large number of adventitious buds and adventitious buds were transferred to 1 / 2MS + IAA 0 .2 ~ 0 .3mg · L- 1 or 1 / 2MS + NAA 0.2mg · L- 1 medium, rapid growth of buds, leaf growth, and you can see adventitious root differentiation, and so on Get strong growth of test tube seedlings will root bud robust plantlets transplanted out of the bottle, the normal development of seedlings.Transplanting times that keep the air around the seedlings moist, good ventilation of the cultivation medium is the key to the success of transplanting.