论文部分内容阅读
目的研究壬基酚(nonylphenol,NP)对秀丽隐杆线虫的生殖毒性。方法将秀丽隐杆线虫暴露于终浓度分别为含0(溶剂对照)、0.01、0.1、1、10、100mg/LNP的培养基24 h,并设空白对照。检测NP对线虫的半数致死浓度(LC50)、后代数目、世代时间和精子发生相关基因spe-10、spe-15、folt-1、fer-1 mRNA的表达水平。结果 NP对线虫的LC50远高于100 mg/L。与溶剂对照组比较,各浓度NP暴露组秀丽隐杆线虫世代时间延长,差异均有统计学意义(P<0.05);而后代数目无明显改变;且随着NP暴露浓度的升高,秀丽隐杆线虫世代时间呈延长趋势,后代数目呈下降趋势。与溶剂对照组比较,仅1、100 mg/L NP暴露组秀丽隐杆线虫spe-10 mRNA的表达水平降低,差异均有统计学意义(P<0.05);而各浓度NP暴露组秀丽隐杆线虫spe-15、folt-1、fer-1 mRNA的表达水平均无明显改变;且随着NP暴露浓度的升高,秀丽隐杆线虫spe-10、folt-1、fer-1 mRNA的表达水平呈下降趋势。结论 NP抑制线虫精子发生相关基因spe-10的表达,对后代数目和世代时间影响较小,本实验结果表明运用线虫模型评价NP的生殖毒性,其基因表达的改变先于表型指标。
Objective To study the reproductive toxicity of nonylphenol (NP) to C. elegans. Methods Caenorhabditis elegans were exposed to the final concentration of 0 (solvent control), 0.01,0.1,1,10,100 mg / LNP medium 24 h, and set a blank control. The median lethal concentration (LC50), the number of progeny, the generation time and the expression of spe-10, spe-15, foll-1 and fer-1 mRNA of nematodes were detected. Results The LC50 of NP to nematodes was much higher than 100 mg / L. Compared with the solvent control group, the Caenorhabditis elegans prolonged for each generation of NP exposure group, the difference was statistically significant (P <0.05), while the number of progeny did not change significantly. With the increase of NP exposure concentration, The age of C. elegans showed an increasing trend, and the number of offspring showed a downward trend. Compared with the solvent control group, the expression level of spe-10 mRNA in C. elegans exposed to only 1,100 mg / L NP exposure was significantly lower than that in the solvent control group (P <0.05) The expression levels of spe-15, folt-1 and fer-1 mRNA did not change significantly in nematode. The expression of spe-10, folt-1 and fer-1 mRNA of C. elegans increased with the increase of NP exposure Showing a downward trend. Conclusion The NP expression of spe-10, a gene related to spermatogenesis in nematode, has little effect on the number of offspring and the passage time. The results of this experiment indicated that the nematode model was used to evaluate the reproductive toxicity of NP, and its gene expression changed earlier than the phenotypic index.