用链佐霉素(STZ)建立2型糖尿病小鼠模型及中药治疗效果的研究

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小白鼠高脂高糖喂养一个月后,用链佐霉素小剂量多次腹腔注射造模。测定造模前后血糖含量。造模成功后将造模小鼠分组,即模型组、治1组和治2组,另设正常组。治2组用舒糖宝治疗,治1组用盐酸二甲双胍治疗(标准药物对照组),模型组和正常组为不治疗组。结果正常组、模型组、治1组、治2组依次为,造模前血糖为6.25±0.92、6.52±1.15、6.90±0.74、6.67±1.22(mmol/L),各组间无显著性差异(P>0.05);造模后血糖为6.12±0.90、14.89±1.44、23.31±6.90、20.52±6.22(mmol/L),正常组明显低于造模组,模型组也明显低于治疗组(P<0.01和<0.05),造模组明显高于造模前,差异有统计学意义,P<0.01;治疗后血糖分别为5.88±0.77、14.01±4.60、15.13±6.46、11.80±4.79(mmol/L);正常组明显低于模型组和治疗组(P<0.01)但治疗组治疗后明显低于治疗前,差异有统计学意义,P<0.01;总胆固醇(TC)分别为1.29±0.29、1.87±0.52、1.62±0.22、1.90±0.67(mmol/L),模型组明显高于正常组(P<0.05);甘油三酯(TG)分别为1.53±0.45、1.17±0.37、0.84±0.26、0.87±0.25(mmol/L),治疗组明显低于正常组(P<0.05和<0.01),治2组低于模型组(P<0.01)。结论治疗后,治1组和治2组血糖均明显下降,甘油三酯降低。 After a month of high-fat and high-sugar mice, they were injected intraperitoneally with low dose of streptozotocin. Determination of blood sugar levels before and after modeling. After the success of modeling, mice will be grouped into groups, namely model group, rule 1 group and rule 2 group, another set normal group. Treatment group 2 Shu Shuang Bao treatment, a group treated with metformin hydrochloride (standard drug control group), model group and normal group as untreated group. Results The blood glucose of normal group, model group, treatment group 1 and treatment 2 group were 6.25 ± 0.92,6.52 ± 1.15,6.90 ± 0.74,6.67 ± 1.22 (mmol / L) before modeling, there was no significant difference among the groups (P> 0.05). After modeling, the blood glucose was 6.12 ± 0.90, 14.89 ± 1.44, 23.31 ± 6.90, 20.52 ± 6.22 (mmol / L) in the normal group and significantly lower than that in the model group P <0.01 and <0.05), and the model group was significantly higher than before modeling, the difference was statistically significant, P <0.01; after treatment, the blood glucose were 5.88 ± 0.77,14.01 ± 4.60,15.13 ± 6.46,11.80 ± 4.79 (mmol / L) in the normal group was significantly lower than that of the model group and the treatment group (P <0.01), but the difference between the two groups was statistically significant (P <0.01); the total cholesterol (TC) was 1.29 ± 0.29 , 1.87 ± 0.52,1.62 ± 0.22,1.90 ± 0.67 (mmol / L), the model group was significantly higher than the normal group (P <0.05); the TGs were 1.53 ± 0.45, 1.17 ± 0.37 and 0.84 ± 0.26 , 0.87 ± 0.25 (mmol / L). The treatment group was significantly lower than the normal group (P <0.05 and <0.01), the treatment group 2 was lower than the model group (P <0.01). Conclusion After treatment, the levels of blood sugar in treatment group 1 and treatment group 2 were obviously decreased, and the level of triglyceride was decreased.
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