AIM:To study the anti-tumor effect of resveratrol and incombination with 5-FU on murine liver cancer.METHODS:Transplantable murine hepatoma_(22) model wasused to evaluate the anti-tumor activity of resveratrol(RES)alone or in combination with 5-FU in vivo.H_(22) cell cycleswere analyzed with flow cytometry.RESULTS:Resveratrol could inhibit the growth of murinehepatoma_(22),after the mice bearing H_(22) tumor were treatedwith 10 mg/kg or 15 mg/kg resveratrol for ten days,and theinhibition rates were 36.3%(n=10)and 49.3%(n=9),respectively,which increased obviously compared with thatin control group(85±22 vs 68±17,P<0.01).RES couldinduce the S phase arrest of H_(22) cells,and increase thepersentage of cells in S phase from 59.1%(n=9)to 73.5%(n=9)in a dose-dependent manner(P<0.05).The enhancedinhibition of tumor growth by 5-FU was also observed inhepatoma_(22) bearing mice when 5-FU was administered incombination with 10 mg/kg resveratrol.The inhibition ratesfor 20 mg/kg or 10 mg/kg 5-FU in combination with 10 mg/kgresveratrol were 77.4% and 72.4%,respectively,comparedwith the group of 20 mg/kg or 10 mg/kg 5-FU alone,in whichthe inhibition rates were 53.4% and 43.8%,respectively(n=8).There was a statistical significance between thecombination group and 5-FU group.CONCLUSION:RES could induce the S phase arrest of H_(22)cells and enhance the anti-tumor effect of 5-FU on murinehepatoma_(22) and antagonize its toxicity markedly.Theseresults suggest that resveratrol,as a biochemical modulatorto enhance the therapeutic effects of 5-FU,may be potentiallyuseful in cancer chemotherapy. AIM: To study the anti-tumor effect of resveratrol and incombination with 5-FU on murine liver cancer. METHODS: Transplantable murine hepatoma (22) model wasused to evaluate the anti-tumor activity of resveratrol (RES) alone or in combination with 5 Resveratrol could inhibit the growth of murine hepatoma (22), after the mice bearing H 22 tumors were treated with 10 mg / kg or 15 mg / kg resveratrol for ten days, and the rates of inhibition were 36.3% (n = 10) and 49.3% (n = 9), respectively, which increased significantly more than that of control group (85 ± 22 vs. 68 ± 17, P <0.01) the S phase arrest of H_ (22) cells, and increase the pace of cells in S phase from 59.1% (n = 9) to 73.5% (n = 9) in a dose- dependent manner tumor growth by 5-FU was also observed in hepaticoma (22) bearing mice when 5-FU was administered incombination with 10 mg / kg resveratrol.The inhibition rates for 20 mg / kg or 10 mg / kg 5-FU in combination with 10 mg / kg resveratrol were 77.4% and 72.4%, respectively, compared with the group of 20 mg / kg or 10 mg / kg 5-FU alone, in which the inhibition rates were 53.4% ​​and 43.8%, respectively (n = 8 ). Here was a statistical significance between the combination group and 5-FU group. CONCLUSION: RES could induce the S phase arrest of H_ (22) cells and enhance the anti-tumor effect of 5-FU on murinehepatoma_ (22) and antagonize its toxicity markedly. Theseresults suggest that resveratrol, as a biochemical modulatorto enhance the therapeutic effects of 5-FU, may be potentially useful in cancer chemotherapy.