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The interest in the analysis of phospholipids(PLs), especially phosphatidylcholine(PC), has been increa- sing due to the importance of them in biochemistry as well as in industry. A method was reported based on the offiine combination of MALDI-TOF MS and normal-phase HPLC for analyzing PLs extracted from crucian carp. Total PLs of crucian carp were extracted and then separated by HPLC before the collected subfractions were analyzed by MALDI-TOF MS. The mass spectra obtained show peaks of H and K