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对一批美国进境的非种用黄大豆和夹带的大豆茎秆进行了病原菌分离,在大豆茎秆上分离到1株疑似葡萄茎枯病菌的菌株Pg-84302。通过对该菌株进行病原菌形态学观察、致病性测定并结合分子生物学方法检测,结果表明菌株Pg-84302在PDA培养基和大豆茎秆上均产生大量分生孢子器、分生孢子和砖格状厚垣孢子,分生孢子器通常为球形;经真菌通用引物扩增和测序,其与GenBank中Phoma glomerata序列(登录号:EU098115.1、AF126819.1、EU273521.1、AY183371.1)的同源性为100%;该菌不仅能侵染大豆,还侵染小麦和苹果。根据上述实验结果,将分离获得的菌株Pg-84302鉴定为葡萄茎枯病菌(Phoma glomerata(Corda)Wollenweber et Hochapfel)。
Pathogenic bacteria were isolated from a number of non-cultivated yellow soybeans and entrained soybean stems imported from the United States, and one Pg-84302 strain was isolated from soybean stalk. By pathogen morphological observation, pathogenicity determination and molecular biology method, the strain Pg-84302 produced a large number of conidia, conidia and bricks on PDA medium and soybean stem The chlamydospore and pycnidia were usually spherical. The common fungi were amplified and sequenced, which shared the same sequence with Phoma glomerata (GenBank accession number: EU098115.1, AF126819.1, EU273521.1, AY183371.1) The homology is 100%; the bacteria can not only infect soybean, but also infect wheat and apple. Based on the above experimental results, the isolated strain Pg-84302 was identified as Phoma glomerata (Corda) Wollenweber et Hochapfel.