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目的研究泛素结合酶E2C(Ubc Hl0)在早期子宫颈癌中的表达及临床意义。方法通过免疫组织化学的方法检测104例子宫颈癌组织、80例子宫颈上皮内瘤变(CIN)组织及30例正常子宫颈组织Ubc H10蛋白的表达情况。并对104例子宫颈癌患者进行随访。结果 Ubc H10在正常子宫颈上皮细胞、CIN及子宫颈癌组织中表达随着病变进展有增强趋势,差异均有统计学意义(P<0.001)。Ubc H10蛋白表达在年龄≤35岁、深间质浸润、宫旁淋巴血管间隙浸润(LVSI)及淋巴转移的肿瘤中表达增强(P<0.05)。单因素分析显示Ubc H10蛋白表达增强、FIGO分期、深间质浸润、LVSI、淋巴转移与子宫颈癌预后相关(P<0.05)。多因素分析显示盆腔淋巴转移、深间质浸润与子宫颈癌预后相关(P<0.05)。结论 Ubc H10参与子宫颈癌的发生和发展。淋巴转移、深间质浸润是早期子宫颈癌独立的预后因素。
Objective To study the expression of ubiquitin-binding enzyme E2C (Ubc Hl0) in early stage cervical cancer and its clinical significance. Methods Immunohistochemistry was used to detect the expression of Ubc H10 protein in 104 cases of cervical cancer, 80 cases of cervical intraepithelial neoplasia (CIN) and 30 cases of normal cervical tissue. 104 cases of cervical cancer patients were followed up. Results The expression of Ubc H10 in normal cervical epithelium, CIN and cervical cancer tissues tended to increase with the progression of the disease, the differences were statistically significant (P <0.001). The expression of Ubc H10 protein was enhanced in tumors of ≤35 years of age, deep interstitial infiltration, parametrial lymphatic vascular space invasion (LVSI) and lymphatic metastasis (P <0.05). Univariate analysis showed that Ubc H10 protein expression increased, FIGO staging, deep stromal infiltration, LVSI, lymphatic metastasis and prognosis of cervical cancer (P <0.05). Multivariate analysis showed that pelvic lymph node metastasis and deep interstitial infiltration were correlated with the prognosis of cervical cancer (P <0.05). Conclusion Ubc H10 is involved in the occurrence and development of cervical cancer. Lymphatic metastasis, deep interstitial infiltration is an independent prognostic factor for early cervical cancer.