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通过十字交叉法和血球计数板法测定了柿树炭疽病菌Collelotrichum gloeosporioides的生物学特性;采用菌丝生长速率法测定了7种常用杀菌剂对该病菌的抑制作用;选择5种市售杀菌剂进行了田间药效试验。结果表明:该病菌菌丝生长的最适温度为20~30℃,最适产孢温度为25~30℃,最适生长的pH值为5~6;在7种常用杀菌剂中,多菌灵、苯醚甲环唑、戊唑醇和氟硅唑对柿树炭疽病菌具有明显的抑制作用,EC50值分别为0.118 8、0.138 0、0.159 9和1.297 2μg/mL。田间试验结果显示,25%咪鲜胺乳油600倍液、10%苯醚甲环唑水分散粒剂1 000倍液和25%戊唑醇水乳剂1 500倍液的防治效果分别为89.60%、88.56%、87.82%。
The biological characteristics of Collelotrichum gloeosporioides were determined by cross method and hemacytometer. The mycelium growth rate method was used to determine the inhibitory effect of seven common fungicides on the pathogen. Five commercially available fungicides were selected Field efficacy test. The results showed that the optimum temperature for mycelium growth was 20-30 ℃, the optimum temperature for sporulation was 25-30 ℃, and the optimum pH for growth was 5-6. Of the seven commonly used fungicides, Chlorpheniramine, difenoconazole, tebuconazole and flusilazole had significant inhibitory effects on anthracnose of Persimmon, with EC50 values of 0.118 8,0.138 0,0.159 9 and 1.297 2 μg / mL, respectively. The results of field trials showed that the control effects of 25% prochloraz EC 600 times, 10% difenoconazole 1000 times and 25% tebuconazole 1500 ml were 89.60% 88.56%, 87.82%.