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目的 研究二氢毗陡类钙桔抗剂，［２，６ 二甲基－４－（３－硝基苯基）－１，４－二氢－３，５－吡啶二羧酸－３－甲酯－５－正戊酯（Ｍｎ９２０２）了、拉西地平（ｌａｃｉｄｉｐｉｎｅ）对培养的大鼠胃底平滑肌 ５－ＨＴ、５－ＨＴ合成酶（ＴＨ）及 ５ＨＴ受体（５－ＨＴＲ）的影响。方法采用免疫细胞化学和图像分析等方法观察Ｍｎ９２０２和ｌａｃｉｄｉｐｉｎｅ作用下培养的大鼠胃底平滑肌细胞中５－ＨＴ、ＴＨ和５－ＨＴＲ含量的变化．并与５－ＨＴ的桔抗剂赛庚定（ｃｙｐｒｏｈｅｔａｄｉｎｅ）比较。结果 正常大鼠胃底平滑肌细胞５－ＨＴ、ＴＨ、５－ＨＴＲ免疫反应均呈阳性，而Ｍｎ９２０２、ｌａｃｉｄｐｉｎｅ和ｃｙｐｒｏｈｅｔａｄｉｎｅ均能使胃底平滑肌５－ＨＴ、ＴＨ和５－ＨＴＲ的含量减少。结论 大鼠胃底平滑肌胞具有自分泌５－ＨＴ的功能，Ｍｎ９２０２和拉西地平可能通过减少５－ＨＴ的合成而使５－ＨＴＲ的表达下降，这一结果与５－ＨＴ桔抗剂赛庚定相似。
OBJECTIVE To study the inhibitory effects of dihydropyropene calcium antagonist, [2,6-dimethyl-4- (3-nitrophenyl) -1,4-dihydro-3,5-pyridinedicarboxylate 5-n-pentyl ester (Mn9202), and lacidipine on 5-HT, 5-HT synthase (TH) and 5HT receptor (5-HTR) in cultured rat gastric fundus. Methods Immunocytochemistry and image analysis were used to observe the changes of 5-HT, TH and 5-HTR in rat gastric fundus smooth muscle cells cultured with Mn9202 and lacidipine. And compared with the 5-HT orange antagonist cyprohetadine. Results The 5-HT, TH and 5-HTR immune responses in normal rat gastric fundus smooth muscle cells were all positive. However, Mn9202, lacidpine and cyprohetadine all reduced the content of 5-HT, TH and 5-HTR in gastric fundus. Conclusions Rat gastric fundus smooth muscle cells have the function of autocrine 5-HT. Mn9202 and lacidipine may reduce the expression of 5-HTR by reducing the synthesis of 5-HT. This result is consistent with that of 5-HT, Set similar.