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目的探讨三七总皂甙(PNS)预处理对大鼠供肝的保护作用及其对供肝细胞凋亡和TNF-α、Caspase-3mRNA表达的影响。方法雄性SD大鼠分别用作供、受体,采用Kamada’s袖套法建立原位肝移植模型,根据供肝切取前1h是否静脉注射PNS(50mg/kg)将大鼠随机分为2组:PNS预处理组(P组)和NS对照组(N组);另设假手术作对照组(S组)。分别于供肝再灌注后2、6、24h处死各组动物,检测血清ALT、AST,HE切片作组织学检查,TUNEL法检测肝细胞凋亡,RT-PCR法检测TNF-α、Caspase-3mRNA的表达。结果供肝再灌注后2、6、24h各时点,P组大鼠血清ALT、AST水平及肝细胞凋亡指数(AI)均明显低于N组(P<0.05);供肝再灌注后2h、6h,P组大鼠肝组织TNF-α、Caspase-3mRNA的相对表达水平明显低于N组(P<0.05)。结论三七总皂甙(PNS)预处理大鼠供肝,可以有效地减轻移植肝的缺血/再灌注损伤和细胞凋亡,影响TNF-α、Caspase-3的表达,可能为PNS抗细胞凋亡的机制之一。
Objective To investigate the protective effect of Panax notoginseng saponin (PNS) preconditioning on donor liver and its effects on hepatocyte apoptosis and expression of TNF-α and Caspase-3 mRNA. Methods Male Sprague-Dawley rats were used as donors and recipients, respectively. Kamada’s cuff method was used to establish orthotopic liver transplantation models. Rats were randomly divided into 2 groups according to whether intravenous PNS (50 mg/kg) was given 1 h before donor liver incision: PNS. Pretreatment group (P group) and NS control group (N group); sham operation was used as control group (S group). Animals were sacrificed 2, 6, and 24 h after reperfusion, and serum ALT and AST were measured. HE slices were taken for histological examination. TUNEL was used to detect hepatocyte apoptosis. RT-PCR was used to detect TNF-α and Caspase-3 mRNA. expression. Results Serum ALT and AST levels and hepatocyte apoptosis index (AI) in group P were significantly lower than those in group N (P<0.05) at the time points of 2, 6, and 24 h after reperfusion; At 2h and 6h, the relative expression levels of TNF-α and Caspase-3 mRNA in the liver of group P were significantly lower than those in group N (P<0.05). Conclusion Panax notoginseng saponin (PNS) pretreatment of rat donor liver can effectively reduce ischemia/reperfusion injury and apoptosis of transplanted liver, and affect the expression of TNF-α and Caspase-3. It may be PNS anti-tumor effect. One of the mechanisms of death.