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目的探讨活性氧分子在奥沙利铂诱导凋亡相关基因PUMA表达致肠癌细胞凋亡过程中的作用及影响。方法采用过氧化氢做为体外氧化应激的模型,WesternBlot检测过氧化氢处理后PUMA蛋白的表达,Hoechst33258荧光染色法检测过氧化氢处理后肠癌细胞的凋亡,MTT法检测在联合应用奥沙利铂和抗氧化剂后肠癌细胞的增殖率。结果一定量的过氧化氢可以诱导肠癌细胞的凋亡及PUMA的表达;抑制PUMA的表达可以减小过氧化氢诱导的肠癌细胞的凋亡;抗氧化剂可以减弱奥沙利铂对肠癌细胞中PUMA表达的诱导;抗氧化剂可以增加奥沙利铂处理后肠癌细胞的增殖率。结论PUMA在氧化应激诱导的肠癌细胞的凋亡过程中具有重要作用;氧化应激在奥沙利铂诱导肠癌细胞凋亡的过程中具有一定作用;奥沙利铂可能部分通过增加活性氧分子的产生而诱导PUMA的表达,从而诱导肠癌细胞发生凋亡。
Objective To investigate the role of reactive oxygen species (ROS) in oxaliplatin-induced apoptosis of colorectal cancer cells induced by PUMA. Methods Hydrogen peroxide was used as a model for in vitro oxidative stress. The expression of PUMA protein was detected by Western blotting and the apoptosis was detected by Hoechst33258 fluorescence staining. The MTT assay was used to detect Proliferative rate of Sariplatin and anti-oxidant humor cells. Results A certain amount of hydrogen peroxide could induce the apoptosis of colorectal cancer cells and the expression of PUMA. Suppression of PUMA expression could reduce the apoptosis of hepatocellular carcinoma cells induced by hydrogen peroxide. Antioxidant could attenuate the inhibitory effect of oxaliplatin on colon cancer Induction of PUMA expression in cells; Antioxidants can increase the proliferation of colorectal cancer cells treated with oxaliplatin. Conclusions PUMA plays an important role in the process of apoptosis induced by oxidative stress. Oxidative stress may play a role in oxaliplatin-induced apoptosis of human colorectal cancer cells. Oxaliplatin may be partly mediated by increased activity Oxygen molecules induced the expression of PUMA, thereby inducing apoptosis in colorectal cancer cells.