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目的:探讨不同剂量环磷酰胺对小鼠肿瘤成模情况的影响,寻找一种简单、有效的建立肿瘤模型的预处理方法。方法:给予BALB/c裸鼠腹腔注射不同剂量环磷酰胺,72小时后再给予小鼠皮下接种淋巴瘤细胞,观察预处理前后小鼠外周血白细胞数量及体重变化情况,以及肿瘤成模率、急性死亡率等。结果:①组1(NS对照组)、组2(100mg/kg×1d)、组3(125mg/kg×1d)、组4(75mg/kg×2d)预处理后体重较处理前无显著性变化,亦无急性死亡情况发生;而组5(125mg/kg×2d)、组6(200mg/kg×2d)、组7(125mg/kg×3d)、组8(250mg/kg×3d)小鼠体重较预处理前明显减轻,且急性死亡率依次为30%、58.3%、50%、75%;②组1和组2小鼠预处理后72小时外周血白细胞数较处理前无明显差异,同时均未成模;而组3、组4、组5、组6、组7、组8小鼠白细胞较预处理前均显著下降,成模率依次为20%、83.3%、60%、33.3%、50%、25%。结论:使用环磷酰胺75mg/kg连续2天腹腔注射的预处理方案,操作简单,成本低廉,通过观察外周血白细胞数和小鼠体重水平等指标即可初步判断建模情况,同时肿瘤成模率高,毒副作用小,是理想的预处理方案。
OBJECTIVE: To investigate the effect of different doses of cyclophosphamide on tumor formation in mice and to find a simple and effective pretreatment method for establishing a tumor model. Methods: BALB / c nude mice were injected intraperitoneally with different doses of cyclophosphamide. The mice were subcutaneously inoculated with lymphoma cells 72 hours later. The changes of peripheral blood leukocytes and body weight were observed before and after pretreatment. The rates of tumor formation, Acute mortality and so on. Results: The body weight in group 1 (NS control group), group 2 (100mg / kg × 1d), group 3 (125mg / kg × 1d) and group 4 (75mg / kg × 2d) And no acute death occurred in group 5 (125mg / kg × 2d), group 6 (200mg / kg × 2d), group 7 (125mg / kg × 3d) and group 8 The body weight of rats in group 1 and group 2 were significantly lower than that before pretreatment, and the acute death rates were 30%, 58.3%, 50% and 75% respectively. ② The number of peripheral white blood cells in group 1 and group 2 was no significant difference , And none of the three groups had a significant decrease in leukocyte pre-treatment compared with that of pre-treatment in groups 3, 4, 5, 6, 7, and 8, and the rates of forming rates were 20%, 83.3%, 60% and 33.3 %, 50%, 25%. CONCLUSION: The pretreatment regimen of intraperitoneal injection of cyclophosphamide (75mg / kg) for 2 consecutive days is simple and cost-effective. Preliminary observation can be made on the modeling by observing the peripheral blood leukocytes and the body weight of mice. At the same time, High rate, small side effects, is the ideal pretreatment program.