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目的:应用基因芯片技术探讨“劳倦过度、房室不节”肾阳虚模型小鼠睾丸生殖相关基因的改变,从基因水平上探讨肾阳虚证和“肾主生殖”的本质。方法:连续4周雄雌鼠按比例1:6同笼喂养,每天更换动情期雌鼠,雄鼠每日游泳,建立“劳倦过度、房室不节”肾阳虚小鼠模型。用小鼠全基因组Oligo芯片检测对照组和模型组睾丸基因,以二者荧光信号相对强度比值≥2和≤0.5筛选差异表达基因,查阅北京博奥生物分子注释系统,进行基因功能分类。结果:成功建立“劳倦过度、房室不节”肾阳虚小鼠模型,筛选出大量差异表达基因,其中上调2425个,下调3080个。上调基因前百位中已知基因41个,下调基因前百位中已知基因62个,主要涉及细胞结构/功能、物质/能量代谢、信号转导/传递、炎症/免疫、细胞凋亡、转录/翻译、增殖/分化及细胞周期等。结论:模型小鼠出现与生殖细胞增殖、凋亡、精子发生和运动、能量代谢相关的基因改变,揭示了肾阳虚证及“肾主生殖”的部分实质。
OBJECTIVE: To investigate the changes of reproductive-related genes in testis of mice with “overdrive and atrioventricular node” model by gene chip technique and to explore the nature of kidney-yang deficiency syndrome and “kidney reproductive system” from the gene level . Methods: Male and female rats were fed 1: 6 in proportion for 4 consecutive weeks, and their estrus females and males were daily swaped daily to establish a mouse model of “overworked and atrioventricular” “kidney yang deficiency. The whole genome of mouse Oligo was used to detect the testis gene of the control group and the model group. The relative expression of the two fluorescent signals was screened by the relative intensity ratio of ≥2 and ≤0.5, respectively. The differential expression genes were screened by Beijing Boao Biomolecular Annotation System for gene function classification. Results: We successfully established a mouse model of ”excessive fatigue, atrioventricular node" kidney deficiency and screened a large number of differentially expressed genes, of which 2425 were up-regulated and 3080 were down-regulated. There were 41 known genes and 62 known genes down-regulated in the first hundred genes, which mainly involved in cell structure / function, substance / energy metabolism, signal transduction / transmission, inflammation / immunity, apoptosis, Transcription / translation, proliferation / differentiation and cell cycle. Conclusion: The model mice showed gene changes related to germ cell proliferation, apoptosis, spermatogenesis and exercise, and energy metabolism, revealing part of the essence of kidney yang deficiency and kidney main reproductive.