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甲基丙烯酸环氧丙酯 (GMA)导致人胚肺成纤维细胞 (HELFs)恶性转化及其潜在致癌机理尚未阐明 .本研究用 0 .5- 5.0 mg· L-1的 GMA给体外培养的 HELFs染毒 2 h和 1 2 h,或用 5.0 mg· L-1的 GMA染毒 1 5min- 2 4 h,应用单细胞凝胶电泳技术 (彗星试验 )对 GMA引起的 HELFs DNA断裂作用进行了初步探讨 .琼脂糖凝胶电泳及流式细胞仪检测等方法观察了 GMA对细胞凋亡的诱导作用 .结果表明 ,GMA可导致染毒细胞 DNA发生剂量和时间依赖性链断裂 .用 5.0 mg· L-1的 GMA染毒后仅 1 h断裂作用即已显著 ,并随染毒时间延长而递增 ,至 2 4 h时损伤最为严重 .但在同样条件下未观察到 GMA对细胞凋亡的诱导作用 .结果提示 GMA导致的非凋亡性 DNA链断裂可能是其诱导 HELFs恶性转化早期重要的遗传事件之一
The malignant transformation of human embryonic lung fibroblasts (HELFs) induced by glycidyl methacrylate (GMA) and its underlying carcinogenic mechanism have not been elucidated.In this study, GMA with 0.5-5.0 mg · L -1 was used to in vitro culture HELFs The DNA damage of GMA-induced HELFs DNA was preliminarily investigated by single cell gel electrophoresis (comet assay) after exposure to 2 and 12 h of exposure or exposure to GMA at 5.0 mg · L -1 for 15 min to 24 h The effect of GMA on apoptosis was observed by agarose gel electrophoresis and flow cytometry.The results showed that GMA could induce dose-and time-dependent DNA strand breaks in the infected cells.With 5.0 mg · L -1 GMA after 1 h fracture was significant, and with the extension of the exposure time and increased to 24 h when the most serious injury, but under the same conditions did not observe the induction of apoptosis of GMA The results suggest that GMA-induced non-apoptotic DNA strand breaks may be one of the important genetic events that induce early malignant transformation of HELFs