用PCR对细胞内和体液中爱滋病毒－1DNA和RNA准确定量现在被认为是研究爱滋病毒-1致病和抗逆转录病毒药物疗效的重要手段。最初若干研究者用半定量PCR技术检测外周血单核细胞（PBMC）和血浆中HIVDNA和RNA水平，但其不能控制每个标本的逆转录和DNA扩增效率。近来报道了定量竞争PC The accurate quantification of HIV-1 DNA and RNA in cells and bodily fluids by PCR is now considered as an important means of investigating the efficacy of HIV-1 pathogenic and antiretroviral drugs. Several researchers initially used semi-quantitative PCR to detect HIVDNA and RNA levels in peripheral blood mononuclear cells (PBMCs) and plasma but failed to control the reverse transcription and DNA amplification efficiency of each sample. Recently reported quantitative competition PC