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目的 :检测正常子宫内膜、子宫内膜息肉及子宫内膜癌中雌激素受体β(estrogen receptorβ,ERβ)基因启动子区Cp G岛甲基化状态,及以上组织中Survivin、Ki-67的表达,探讨其中ERβ启动子甲基化与Survivin、Ki-67表达的关系,分析其中的联系,探讨子宫内膜息肉组织的增生和细胞凋亡机制。方法:运用甲基化特异性PCR(MSP)检测40例正常子宫内膜、40例子宫内膜息肉组织及26例子宫内膜癌组织ERβ甲基化情况,同时采用免疫组化ELIVISONTM二步法检测以上组织中Survivin、Ki-67基因蛋白的表达。结果:正常子宫内膜及子宫内膜息肉组织中ERβ基因甲基化较子宫内膜癌组织中ERβ基因甲基化显著降低(P<0.05)。正常子宫内膜组织中Survivin表达较子宫内膜息肉组织与子宫内膜癌组织的Survivin蛋白表达显著降低(P<0.05)。正常子宫内膜及子宫内膜息肉中Ki67表达较子宫内膜癌组织中明显降低(P<0.05)。子宫内膜息肉及子宫内膜癌组织中Survivin及Ki67阳性表达与ERβ基因启动子甲基化均呈正相关。结论:ERβ基因甲基化是子宫内膜息肉发生的影响因素之一,子宫内膜组织中ERβ基因甲基化可能与子宫内膜息肉的产生、细胞增殖、复发和进一步发展及预后密切相关。
OBJECTIVE: To detect the methylation status of CpG island in promoter region of estrogen receptorβ (ERβ) gene in normal endometrium, endometrial polyps and endometrial carcinoma, and to investigate the expression of Survivin, Ki-67 The relationship between methylation of ERβ promoter and the expression of Survivin and Ki-67 was explored. The relationship between ERβ promoter methylation and Ki-67 expression was analyzed. The mechanism of hyperplasia and apoptosis of endometrial polyps was explored. Methods: The methylation-specific PCR (MSP) was used to detect the ERβ methylation in 40 cases of normal endometrium, 40 cases of endometrial polyps and 26 cases of endometrial carcinoma. The immunohistochemical ELIVISONTM two-step method Detection of Survivin, Ki-67 gene protein expression in the above tissues. Results: Methylation of ERβ gene in normal endometrium and endometrial polyps was significantly lower than that in endometrial carcinoma (P <0.05). The expression of Survivin in normal endometrium was significantly lower than that in endometrial polyps and endometrial carcinoma (P <0.05). The expression of Ki67 in normal endometrium and endometrial polyps was significantly lower than that in endometrial carcinoma (P <0.05). The positive expression of Survivin and Ki67 in endometrial polyps and endometrial carcinoma were positively correlated with ERβ gene promoter methylation. Conclusion: Methylation of ERβ gene is one of the influencing factors of endometrial polyps. Methylation of ERβ gene in endometrial tissue may be closely related to the production of endometrial polyps, cell proliferation, recurrence and further development and prognosis.