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目的:探讨莲房原花青素(lotus seedpod procyanidins,LSPC)对D-半乳糖衰老小鼠脑组织抗氧化系统的影响。方法:雄性健康昆明种小鼠60只,随机分为对照组、衰老模型组、LSPC低剂量组(15 mg·ml~(-1))、LSPC中剂量组(30 mg·ml~(-1))和LSPC高剂量组(60 mg·ml~(-1))。采用小鼠皮下注射D-半乳糖(120 mg·kg~(-1)·d~(-1)),连续注射8周,建立小鼠衰老模型,LSPC组造模同时灌胃给予LSPC,qd,连续给药8周。化学比色法检测5组小鼠脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)含量。结果:衰老模型组与对照组相比,小鼠脑组织SOD和GSH-Px活性明显降低,MDA含量明显增高(P<0.01)。与衰老模型组相比较,LSPC能明显提高衰老小鼠脑组织中SOD活性和GSH-Px活性(P<0.01),能减少MDA含量(P<0.01)。结论:LSPC对D-半乳糖所致衰老小鼠具有明显的抗氧化作用。
Objective: To investigate the effect of lotus seedpod procyanidins (LSPC) on the anti-oxidation system in brain of D-galactose-induced senile mice. METHODS: Male healthy Kunming mice were randomly divided into control group, aging model group, low dose of LSPC group (15 mg·ml -1), middle dose of LSPC (30 mg·ml -1 )) and LSPC high-dose group (60 mg·ml -1). The mice were injected subcutaneously with D-galactose (120 mg·kg -1 ·d -1) for 8 weeks to establish an aging mouse model. The LSPC group was modeled and intragastrically given LSPC, qd. , continuous administration for 8 weeks. The content of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in brain tissue of five groups of mice were detected by chemical colorimetry. Results: Compared with the control group, the activity of SOD and GSH-Px in the brain of the aging model group was significantly decreased, and the content of MDA was significantly increased (P<0.01). Compared with the aging model group, LSPC significantly increased SOD activity and GSH-Px activity in the brain of aging mice (P<0.01), and decreased MDA content (P<0.01). CONCLUSION: LSPC has significant antioxidant effects on aging mice induced by D-galactose.