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提出了一种基于微纳光纤耦合器的高灵敏度、便携式生物传感器,并对该传感器用于无标生物检测的灵敏度和重复性进行了研究。通过熔融拉锥法拉制出腰椎直径为3μm的光纤耦合器,并进行折射率检测实验。实验测得折射率灵敏度为1402.3nm/RIU,对检测结果进行拟合,得到拟合曲线的相关系数为0.99459。微纳光纤耦合器的弱耦合模型的计算结果和实验结果相符。将此微纳光纤耦合器用于检测羊免疫球蛋白(IgG)抗原,得到了2pg/mL的检出限;10次解离再生实验验证了该传感器的重复性,表明了微纳光纤耦合器在无标生物传感中具有高检测灵敏度的潜力和较好的实用价值。
A highly sensitive and portable biosensor based on the micro-nanofiber coupler was proposed. The sensitivity and repeatability of the sensor for the bioassay without bioburden were also studied. A fiber coupler with lumbar vertebra diameter of 3μm was drawn by melt taper method, and the refractive index test was carried out. The measured refractive index sensitivity was 1402.3nm / RIU, the test results were fitted, the fitting curve obtained correlation coefficient of 0.99459. The calculation results of the weak coupling model of the micro-nano fiber coupler are consistent with the experimental results. The micronaire coupler was used to detect goat immunoglobulin (IgG) antigen, and the detection limit of 2 pg / mL was obtained. The repeatability of the sensor was verified by 10 dissociation regeneration experiments. The potential and good practical value of non-standard bio-sensing with high detection sensitivity.