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以细胞培养法并用肝素等作对照，观察类肝素对兔主动脉血管平滑肌细胞（ＶＳＭＣｓ）增生的影响。结果表明，类肝素在１６～００５ｍｇ·ｍｌ－１浓度下，对ＦＣＳ，ｂＦＧＦ，ＩＬ１刺激细胞增殖均有明显的抑制作用。将ＶＳＭＣｓ在类肝素０２～００５ｍｇ·ｍｌ－１中预孵２４ｈ后弃去药液，再在含ＦＣＳ培养液中继续培养，也能明显抑制细胞增殖。类肝素对ＦＣＳ及ＩＬ１诱导ＶＳＭＣｓ增殖的抑制作用比同浓度的肝素弱，但对ｂＦＧＦ诱导ＶＳＭＣｓ增殖的抑制作用比肝素强。右旋糖酐对ＶＳＭＣｓ增殖无抑制作用，提示抑制作用与分子中含硫酸基有关。
The effects of heparins on proliferation of rabbit aortic vascular smooth muscle cells (VSMCs) were observed by cell culture and heparin. The results showed that heparin at 1 6 ~ 0 05mg · ml-1 concentrations, FCS, bFGF, IL 1 stimulated cell proliferation were significantly inhibited. The VSMCs were preincubated for 24 h in heparinoid 0 2 ~ 0 05 mg · ml-1 and then discarded after further incubation in FCS-containing medium, but also significantly inhibited cell proliferation. Compared with the same concentration of heparin, the inhibitory effect of heparin on FCS and IL-1 induced VSMCs proliferation was weaker than that of heparin on bFGF-induced proliferation of VSMCs. Dextran had no inhibitory effect on VSMCs proliferation, suggesting that the inhibitory effect was related to the presence of sulfate groups in the molecule.