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目的探讨肿瘤细胞的甲硫氨酸依赖性在提高肿瘤化疗疗效方面的作用及意义。方法将甲硫氨酸（Ｍｅｔ）依赖性肿瘤细胞ＳＧＣ７９０１、ＭＣＦ７和非Ｍｅｔ依赖性肿瘤细胞Ａ５４９、Ａ３７５培养于Ｍｅｔ－Ｈｃｙ＋和Ｍｅｔ＋Ｈｃｙ－培养基中，加入不同浓度的周期特异性化疗药５Ｆｕ或非周期特异性化疗药ＭＭＣ；在细胞与药物接触不同时间段，用ＭＴＴ比色法检测化疗药物的抑瘤率。结果Ｍｅｔ－Ｈｃｙ＋中的５Ｆｕ对Ｍｅｔ依赖性肿瘤细胞的抑瘤率较Ｍｅｔ＋Ｈｃｙ－中的５Ｆｕ的抑瘤率有显著性提高；而５Ｆｕ对非Ｍｅｔ依赖性肿瘤细胞的抑瘤率，在两种培养基间差异无显著性。ＭＭＣ对Ｍｅｔ依赖性、非Ｍｅｔ依赖性肿瘤细胞的抑瘤率未因培养基的不同而差异有显著性。结论利用肿瘤细胞的Ｍｅｔ依赖性，Ｍｅｔ－Ｈｃｙ＋培养基可显著提高周期特异性化疗药（５Ｆｕ）对Ｍｅｔ依赖性肿瘤细胞的抑瘤率，但不能提高周期非特异性化疗药（ＭＭＣ）的抑瘤率。
Objective To investigate the role and significance of methionine-dependent tumor cells in improving the efficacy of chemotherapy for tumors. Methods Met-dependent tumor cells SGC-7901, MCF-7 and non-Met-dependent tumor cells A549 and A375 were cultured in Met-Hcy+ and Met+Hcy-medium, and different concentrations of cycle-specific chemotherapy were added. Drug 5 Fu or non-cycle specific chemotherapy drug MMC; in different periods of cell-drug exposure, the inhibition rate of chemotherapeutic drugs was measured by MTT colorimetry. Results The inhibitory rate of 5Fu in Met-Hcy+ against Met-dependent tumor cells was significantly higher than that of 5Fu in Met+Hcy-, while the inhibitory effect of 5Fu on non-Met-dependent tumor cells was significant. The rate was not significantly different between the two media. The inhibition rate of MMC on Met-dependent, non-Met-dependent tumor cells was not significantly different from that of the culture medium. Conclusion Met-Hcy+ medium can significantly increase the tumor inhibition rate of Met-dependent tumor cells in the Met-Hcy+ medium, but it can not increase the non-specific chemotherapy drug (MMC). Inhibitory rate.