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甲状腺C细胞用苏木精-伊红染色时,其细胞质内的分泌颗粒不着色,呈透明状态,需采用特殊染色或银浸法来显示。Blaher(1972)的硝酸银-schiff剂结合法,操作复杂故使用较少。Cajal第三法使用较多,但亦有其不足,正如王建新(1982)改良法所述,其改良法较好,但浸银时需严格控制在20℃,并使用高浓度硝酸银,切片尚需经吡啶处理,操作仍较复杂。Solcia(1968)的天青A法和Petko(1974)的新美蓝法的缺点是染色后经酒精脱水时,C细胞颗粒极易褪色,细胞核无色,不能观察到甲状腺的完整结构,故不适合制作教学标本。Solcia对胰岛A和D细胞,胃肠道G和Ec细胞和甲状腺C细胞用12种液体固定以及固定的时间与水解的时间进行研究,结果表
When thyroid C-cells are stained with hematoxylin-eosin, the secretory granules in the cytoplasm are not colored and are in a transparent state and need to be displayed by special staining or silver immersion. Blaher (1972) silver nitrate-schiff agent binding method, the operation is complicated to use less. The third method of Cajal is more used, but also has its disadvantages. As mentioned in Wang Jianxin’s (1982) Improvement Law, the improved method is better. However, when the silver immersion is strictly controlled at 20 ° C, high concentrations of silver nitrate and slices Still need to be pyridine treatment, the operation is still more complicated. The shortcomings of Azure A method and Petko (1974) method of Solcia (1968) are that C-cell pellet is easily faded when dyed by alcohol, and the nucleus is colorless and can not observe the complete structure of the thyroid gland. Therefore, Suitable for making teaching specimens. Solcia studied the timing of immobilization and fixation of islets A and D cells, gastrointestinal G and Ec cells and thyroid C cells with 12 fluids and the time of hydrolysis, and the results of the table