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目的依据孕妇血浆中存在游离胎儿DNA;;的理论,从孕妇外周血浆中分离出胎儿DNA;;并加以鉴定,探讨晚孕期和临产期母血中胎儿DNA;;含量的变化及其临床意义。方法采用实时荧光定量PCR(real-time fluorescence quantitativepolymerase chain-reaction,FQ-PCR)方法对孕28-42周以及临产时80例孕妇血浆中胎儿DNA;;的含量进行检测并比较,初步探讨游离fDNA;;在不同妊娠状态下的浓度变化。结果孕28～31周组、孕32～35周组、孕36～42周组、临产组孕妇血浆中DNA;;的含量拷贝数分别为(23034±25.52)、(480.08±55.47)、(600.82±70.47)、(1100.34±87.95)copys/mI。各组问孕妇外周血中胎儿DNA;;的含量比较,差异有统计学意义(P<0.05)。结论随着孕期的进展,母血中胎儿DNA;;的量也在逐渐增加,母血中胎儿DNA;;含量的升高可能是分娩的诱因之一。
Objective To separate fetus DNA from pregnant women’s peripheral plasma according to the theory of free fetal DNA in pregnant women’s plasma and to identify the changes of fetus DNA in maternal blood during late pregnancy and labor and its clinical significance. Methods FQ-PCR method was used to detect and compare the fetal DNA content in plasma from 28 pregnant women at 28-42 weeks of pregnancy and at the time of labor. The free fDNA ;; Concentration changes in different pregnancy states. Results The copy number of DNA in plasma of pregnant women was (23034 ± 25.52), (480.08 ± 55.47) and (600.82), respectively, in pregnant women at 28-31 weeks, pregnant 32-35 weeks, pregnant 36-42 weeks, ± 70.47), (1100.34 ± 87.95) copys / mI. Pregnant women asked each group of fetal peripheral blood fetal DNA ;; content comparison, the difference was statistically significant (P <0.05). Conclusion With the progress of pregnancy, the amount of fetal DNA in maternal blood is also gradually increased, fetal DNA in maternal blood; elevated levels may be one of the causes of childbirth.