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土壤细菌ps-5产胞外多糖经乙醇沉淀、Sevage法脱蛋白、透析、冷冻干燥等方法分离和纯化后得到粗多糖,多糖含量为0.3 mg/mL。该多糖对.OH和O2-.有较强的清除能力,当清除率达50%时所需浓度分别为0.0476、0.2770 mg/mL,而在同等条件下,使用Vc清除率达到50%所需浓度分别为0.0440、5.70 mg/mL。研究多糖对油脂的抗氧化活性,结果表明,ps-5胞外多糖可有效抑制动植物油脂的氧化。
The crude polysaccharides were isolated and purified from soil bacterial ps-5 extracellular polysaccharides by ethanol precipitation, Sevage deproteinization, dialysis, freeze-drying and other methods. The content of polysaccharides was 0.3 mg / mL. The polysaccharide has strong scavenging ability to .OH and O2-. When the clearance rate reaches 50%, the required concentrations are 0.0476 and 0.2770 mg / mL, respectively. Under the same conditions, the clearance rate of Vc reaches 50% The concentrations were 0.0440 and 5.70 mg / mL, respectively. The antioxidant activities of polysaccharides on oils and fats were studied. The results showed that ps-5 exopolysaccharide can effectively inhibit the oxidation of animal and plant oils.