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鼓膜修补的取材,最早期使用皮肤移植术来修复鼓膜,移植表皮虽有成功病例,但因穿孔率高,现已基本舍弃。近年来推崇中胚层移植材料,其代谢率低,手术成功机会多,按其来源分自体和异体两种,我院为了解中胚层移植物修补的愈合机制,经动物实验观察,证明无论中胚层组织有无活性其愈合机理一样,均可起支架作用。材料与方法选用自身组织(耳屏软骨膜、颞肌筋膜、耳垂脂肪、静脉)和异体组织(脐带动脉、鼻中隔软骨膜、大隐静脉、鼓膜)。异体组织通过甲醛处理方法。盐水洗涤后放在4％甲醛溶液磷酸盐缓冲液(pH
Tympanic membrane repair drawing, the earliest use of skin grafts to repair the tympanic membrane, although the successful case of transplanted epidermis, but due to high rate of perforation has been basically abandoned. In recent years, respected mesoderm transplantation materials, the metabolic rate is low, the chances of successful surgery, according to their sources of autologous and heterologous two, our hospital in order to understand the healing mechanism of mesoderm graft repair, animal experiments show that both mesoderm Tissue activity is the same as the healing mechanism, can play a role of stent. MATERIALS AND METHODS The tissues (tragus cartilage, temporal fascia, earlobe fat, veins) and allogeneic tissues (umbilical artery, septal perichondrium, saphenous vein, tympanic membrane) were selected. Allogeneic tissue by formaldehyde treatment method. Saline was washed in 4% formaldehyde solution in phosphate buffer (pH