依据慢性粒细胞白血病BCR/ABL融合基因的碱基序列,设计了一种新型发夹结构锁核酸(locked nu-cleic acids,LNA)探针,将该探针借助Au—S键固定在金电极表面构建了特异的生物传感器.LNA探针与目标链DNA杂交,以本实验室合成的苯甲酸二聚铜配合物([Cu2(C7H5O2)4(C2H6O)2],简称[Cu(R)2]2+)为杂交指示剂,应用差示脉冲伏安法进行检测,表现出良好的响应信号.该新型锁核酸传感器能较好的区分完全互补链DNA、单碱基错配链DNA.互补链DNA检测的线性范围为1.0×10-8～1.0×10-6 mol.L-1,检出限2.0×10-9mol.L-1. According to the base sequence of the BCR / ABL fusion gene of chronic myeloid leukemia, a novel locked nu cle cle-acids (LNA) probe was designed. The probe was immobilized on the gold electrode (Cu2 (C7H5O2) 4 (C2H6O) 2], abbreviated as [Cu (R) 2], was synthesized by using the LNA probe hybridized with the target DNA. ] 2+) is a hybridization indicator, which is detected by differential pulse voltammetry and shows a good response signal. The novel locked nucleic acid sensor can better distinguish between DNA of complete complementary strand DNA and single base mismatch DNA The linear range of DNA detection was 1.0 × 10-8 ~ 1.0 × 10-6 mol.L-1, and the detection limit was 2.0 × 10-9mol.L-1.